感染
发表于 2025-3-30 09:19:21
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instill
发表于 2025-3-30 15:29:52
High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse,nts and the role they play in the regulation and execution of NK cell directed secretion. Here we describe a protocol for the recapitulation of the NK cell lytic synapse on glass, the acquisition of microscopy images, and suggested approaches for the processing and analysis of microscopy data.
labile
发表于 2025-3-30 19:04:47
Tracking Effector and Memory NK Cells During MCMV Infection,s with MCMV. Here we describe an adoptive transfer model in which the expansion, contraction, and memory cell persistence of transferred Ly49H. NK cells are tracked in congenic C57BL/6 hosts following MCMV infection.
宏伟
发表于 2025-3-30 21:22:52
Assessment of NK Cell Metabolism, assessment of real-time glycolysis and oxidative phosphorylation by an extracellular flux assay from Seahorse Biosciences, and some basic protocols for stimulation of NK cells via cytokines and receptors.
伟大
发表于 2025-3-31 02:06:26
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ostrish
发表于 2025-3-31 06:26:26
Book 2016new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study
infarct
发表于 2025-3-31 09:45:55
Ex Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21,K562 mb.IL21). We had previously demonstrated that this platform enables the highest fold expansion of NK cells reported in the literature to date (>47,000-folds in 21 days), and produces highly activated and pure NK cells without signs of senescence, as determined by telomere shortening.
矛盾
发表于 2025-3-31 16:41:04
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Abrade
发表于 2025-3-31 19:46:03
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特别容易碎
发表于 2025-3-31 22:49:11
Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry,pecific NK cell subsets is possible. Here, a flow cytometric method for dynamic measurements of intracellular Ca. concentrations in human NK cells subsets is detailed and discussed. This assay can be further adapted for specific scientific and diagnostic questions, with implications for various immunopathological conditions.