傲慢人
发表于 2025-3-25 04:24:30
Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience, at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss s
懒惰民族
发表于 2025-3-25 10:16:13
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garrulous
发表于 2025-3-25 15:01:11
Fluorescence Lifetime Imaging Microscopy (FLIM),c images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation sources and time-correlated or time-gated detection. In this review we describe the different mo
山间窄路
发表于 2025-3-25 18:01:09
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crockery
发表于 2025-3-25 21:24:46
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斜坡
发表于 2025-3-26 01:32:58
Spectral Imaging and Linear Unmixing in Light Microscopy,he creation of increasingly complex multicolored samples. A reliable separation of the different fluorescence labels is required for analysis and quantitation, but it is complicated by the significant overlap of the emission spectra. This problem can be addressed on the acquisition and the processin
文艺
发表于 2025-3-26 06:45:35
Tracking Movement in Cell Biology,ve been developed during the last decade. Both strong and weak points of each technique are discussed, together with short description on the actual application. Among these techniques, the optical flow estimation technique is explained in more detail and an example of protein movement analysis is d
道学气
发表于 2025-3-26 10:42:08
Live Cell Spinning Disk Microscopy, disk microscopy will be explained in this chapter and a thorough comparison of the performance of single beam and multi-beam scanning systems is made and illustrated with an example of in vivo imaging in ..
缩短
发表于 2025-3-26 12:50:31
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幸福愉悦感
发表于 2025-3-26 18:53:07
Fluorescence Correlation Spectroscopy with Autofluorescent Proteins, their genetically encoded fusions to cellular proteins. This review discusses recent applications of FCS analysis with and on fluorescent proteins, particularly highlighting chemical and physical properties. Inherent limitations of the presented approaches are discussed in detail and strategies for optimisation of experimental systems outlined.