Genteel 发表于 2025-3-25 03:48:48
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Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique,in pronuclear injection. Moreover, Cytochalasin B that is used to soften zygotes during piezo-driven microinjection has been suggested a role in improving the knockin efficiency, which provides an additional benefit to use this injection method.吊胃口 发表于 2025-3-26 01:45:46
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The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Speathione to the fertilization medium. These modifications greatly enhanced the fertility of frozen-thawed C57BL/6 sperm, resulting in a stable fertilization rate >80% in IVF. Our results indicate that this robust protocol for sperm cryopreservation may improve the archiving and distributing system foAnticlimax 发表于 2025-3-26 14:07:22
Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line,and single hepatocytes (both genome-edited and wild-type) can be isolated for analysis immediately following transient gene editing. Previously, we demonstrated successful isolation of genome-edited single hepatocytes, using mice expressing systemic Cas9 transgene (called “sCAT” mouse) and by tail-v强壮 发表于 2025-3-26 18:01:35
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