Monocle 发表于 2025-3-28 17:20:07
MicroRNA Identification, Target Prediction, and Validation for Crop Improvement,h, the isolation of miRNAs is a tedious and difficult task using in vitro methods. However, recent exploration into the remarkably highly conserved nature of nucleotide sequences of miRNAs assists in the identification of miRNAs in plant species through homologous approaches. Here, we describe the iLimpid 发表于 2025-3-28 19:50:39
MicroRNA Detection with CRISPR/Cas, and therapeutic purposes. Their small size and low abundance, however, derive challenges in engineering robust detection tools. To date, multiple detection assays have been developed to achieve highly specific recognition of trace amount of miRNA with state-of-the-art nucleic acid detection and sigCRACY 发表于 2025-3-29 02:11:15
http://reply.papertrans.cn/64/6328/632800/632800_43.png一回合 发表于 2025-3-29 03:47:32
http://reply.papertrans.cn/64/6328/632800/632800_44.png手榴弹 发表于 2025-3-29 09:56:13
http://reply.papertrans.cn/64/6328/632800/632800_45.png耕种 发表于 2025-3-29 15:03:44
Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification,we detail a protocol for digital, isothermal, and multiplex detection of microRNAs, which relies on a recently developed amplification method. Our approach uses programmable ultrasensitive molecular amplifiers (PUMAs) to reveal the presence of target microRNAs randomly isolated in picoliter-size micFierce 发表于 2025-3-29 18:20:13
http://reply.papertrans.cn/64/6328/632800/632800_47.pnggustation 发表于 2025-3-29 23:16:59
http://reply.papertrans.cn/64/6328/632800/632800_48.pngSynapse 发表于 2025-3-30 01:55:57
http://reply.papertrans.cn/64/6328/632800/632800_49.png一美元 发表于 2025-3-30 05:02:52
Determining miRNA Expression Patterns in Xenopus,by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in . or . by WISH using two different approaches: LNA-WISH to visualize mature