BRUNT
发表于 2025-3-28 14:49:28
Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polypf proteins and peptides. As a benefit of this distinction, over the last four decades the method has been refined to a high degree of perfection. Nevertheless, sequencing with phenyl isothiocyanate (PITC). suffers from a few practical limitations. First, the extinction coefficient of the phenylthioh
障碍物
发表于 2025-3-28 19:39:32
http://reply.papertrans.cn/64/6324/632335/632335_42.png
microscopic
发表于 2025-3-29 01:03:37
http://reply.papertrans.cn/64/6324/632335/632335_43.png
TIGER
发表于 2025-3-29 06:47:56
A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vaporynthesis of immunoreactive peptides. To date, amino(N)-terminal sequencing using the Edman degradation procedure has almost exclusively provided such data. Methodologies for sequencing proteins from their carboxy(C)-termini have remained relatively primitive requiring much protein in return for litt
duplicate
发表于 2025-3-29 10:15:43
Sequencing of Proteins from the C-Terminus our investigations, including the mechanism of the initial activation of the C-terminal carboxyl group and the deliberate modifications of the reactive side-chains of the amino acids with the sequencing reagents. Through the selection of the reagents and reaction conditions used for our sequencing
cliche
发表于 2025-3-29 13:52:18
http://reply.papertrans.cn/64/6324/632335/632335_46.png
包裹
发表于 2025-3-29 18:47:45
Automated C-Terminal Sequencing of Polypeptides Containing C-Terminal Prolinemethod would be analogous and complimentary to the Edman degradation commonly used for N-terminal sequence analysis (Edman, 1950). It would also be invaluable for the sequence analysis of proteins with naturally occurring N-terminal blocking groups, for the detection of post-translational processing
fluffy
发表于 2025-3-29 22:55:46
Tandem Mass Spectrometric Characterization of Modified Peptides and Proteinsture determination. The value of MS derives from the accommodation of mixtures and the possibilities for characterization of modified amino acid residues. Tandem MS in particular is important in addressing both of these issues. The essential features of tandem MS are the promotion of ion fragmentati
finale
发表于 2025-3-30 02:50:13
Direct Mass Spectrometric Analyses for Protein Chemistry Studies laboratory. Protein sequencing methods and mass spectrometry are often plagued with the same problems in that samples are frequently contaminated with other materials. Many buffer components, salts, and solubilizing detergents can interfere or prevent the successful application of both methodologie
instate
发表于 2025-3-30 06:21:24
http://reply.papertrans.cn/64/6324/632335/632335_50.png