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Using Fluorescence Recovery After Photobleaching to Measure Lipid Diffusion in Membranes,orescence recovery after photobleaching measurements may be carried out in vivo, in systems ranging from bacteria to mammalian cells, or in vitro in isolated membrane fragments. Procedures for preparing the biological sample, performing the measurement, and quantitative data analysis are explained.Munificent 发表于 2025-3-29 23:41:05
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Statistical Thermodynamics Through Computer Simulation to Characterize Phospholipid Interactions initions. Next, the choice of thermodynamic ensembles, and the two main options for the generation of a representative sample of configurations: molecular dynamics and Monte Carlo are discussed. The final issue that is dealt with describes the various ways the generated ensembles can be analyzed.Grating 发表于 2025-3-30 05:40:50
Differential Scanning Calorimetry in the Study of Lipid Phase Transitions in Model and Biological Mrmograms is affected by the nature of the lipid phase transition examined. The overall goal is to provide practical guidelines for the development of data-acquisition and data-analysis protocols, which are compatible with the instrumentation available and the nature of the lipid phase transition under investigation.