法律的瑕疵
发表于 2025-3-25 05:47:54
Oscar Gutiérrez-Sanz,Olaf Rüdiger,Antonio L. De Lacey
纵火
发表于 2025-3-25 11:04:09
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Uncultured
发表于 2025-3-25 13:53:54
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胰脏
发表于 2025-3-25 17:32:53
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清晰
发表于 2025-3-26 00:01:32
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Sciatica
发表于 2025-3-26 01:06:27
Cell-Free Synthesis of the H-Cluster: A Model for the In Vitro Assembly of Metalloprotein Metal Cenell-free system in which the biosynthetic pathway for a prosthetic group has been fully or even partially reconstructed enables investigations of the reaction sequence as well as the cofactor itself. As a model for the in vitro assembly of protein-bound metal centers, we describe a procedure for the
ingenue
发表于 2025-3-26 04:50:39
Electrochemistry of Metalloproteins: Protein Film Electrochemistry for the Study of , -Hydrogheir catalytic properties. The enzyme is deposited onto a working electrode tip, and through control of the applied potential the enzyme activity is monitored as electrical current, allowing for direct study of inherent activity as electrons are transferred to and from the enzyme redox center(s). No
cuticle
发表于 2025-3-26 11:53:43
FTIR Spectroscopy of Metalloproteins,d to the overlap of all the peptide bond absorption. Additionally, in many metalloproteins their prosthetic groups have intrinsic ligands or bind substrates/inhibitors that absorb intensively in the infrared. Here, we describe thoroughly several Fourier transform infrared methods for studying struct
BRINK
发表于 2025-3-26 14:01:56
Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman f short-lived intermediates formed during the enzymatic reactions of metalloproteins. Commercially available equipment allows trapping of intermediates within a millisecond to second time scale for low-temperature RR analysis resulting in the direct detection of metal–ligand vibrations and porphyrin
exorbitant
发表于 2025-3-26 19:10:35
A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and norganic users. This technique yields a vibrational spectrum for a specific element, which can be easily interpreted. Furthermore, isotopic labeling allows for site-specific experiments. In this chapter, we discuss how to access specific beamlines, what kind of equipment is used in NRVS, and how the