acolyte 发表于 2025-3-27 00:54:00

A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity Sensing byrecise control of the geometry of growth, and standardization of the measurements. In sum, MLS enables one to quantitatively test, even on long time scales, the effect of the rigidity and the geometry of the environment on the growth of roots and root hair cells, including mechanotransduction to the nucleus.

acetylcholine 发表于 2025-3-27 02:58:26

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变形 发表于 2025-3-27 08:21:31

Quantifying Strain-Sensing Protein Recruitment During Stress Fiber Repairthen either be compared to (Blanchoin, Physiol Rev 94, 235–263, 2014) a known control (e.g., zyxin-GFP) or (Hoffman, Mol Biol Cell 23, 1846–1859, 2012) the pre-damaged stress fiber protein distribution. With this method, strain-sensing proteins and their dynamic association with stress fiber strain sites can be reproducibly measured and compared.

Patrimony 发表于 2025-3-27 09:56:02

Photoresponsive Hydrogels for Studying Mechanotransduction of Cellss incorporation into a polyacrylamide hydrogel to render it photoresponsive. Precise control over the physical properties of the gel allows observation of glioblastoma durotaxis under surface stiffness conditions relevant to the actual brain environment.

Maximize 发表于 2025-3-27 15:54:41

Quantification of Invadopodia Formation and Matrix Degradation Activitygrading proteinases, and apply protrusive forces generated by the actin cytoskeleton, which drive the invasive process. Here, we describe a fluorescent microscopy-based protocol for imaging and quantifying both invadopodia formation and matrix degradation.

索赔 发表于 2025-3-27 21:10:07

Imaging Cell Adhesive Force at the Single Molecule Levely. ITS can be selectively implemented at two imaging modes: a cumulative mode that maps cell adhesive force at a high signal-to-noise ratio even with a low-end fluorescence microscope, and a real-time mode that images the force at the single molecular tension level.

他很灵活 发表于 2025-3-28 00:33:13

1064-3745expertsThis detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features m

不理会 发表于 2025-3-28 03:31:33

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Rebate 发表于 2025-3-28 08:19:43

Measuring Cellular Traction Forces with Micropillar Arrays of cell spreading on the pillars. This technique offers the advantage of high spatial and temporal resolution analyses and constitutes a method to investigate the effect of substrate rigidities on cellular functions.

平项山 发表于 2025-3-28 12:32:18

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查看完整版本: Titlebook: Mechanobiology; Methods and Protocol Ronen Zaidel-Bar Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to