Precursor
发表于 2025-3-25 06:41:59
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CONE
发表于 2025-3-25 09:20:11
Nanoliter-Scale Sample Preparation for Single-Cell Proteomic Analysis Using Glass-Oil-Air-Droplet Copulations. Mass spectrometry (MS)-based proteomics is a promising alternative for quantitative single-cell proteomics. Various techniques are continually evolving to address the challenges of limited sample material, detection sensitivity, and throughput constraints. In this chapter, we describe a
航海太平洋
发表于 2025-3-25 14:16:53
Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling,roteomics is widely applicable and needs special sample preparation methods to achieve deep proteome profiling. This chapter describes protocols for the preparation and application of an easy-to-use and scalable device for processing low-input samples. Protein preconcentration, impurity removal, red
LUCY
发表于 2025-3-25 19:30:28
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AMITY
发表于 2025-3-25 20:07:33
Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale Protobscures cell-to-cell heterogeneity, precluding proteome profiling of single cells and small numbers of cells of interest. To address this issue, in the recent 5 years, there has been a surge of small sample preparation methods developed for robust and effective collection and processing of single c
Condense
发表于 2025-3-26 00:17:22
Spatial Proteomics of Single Hepatocytes with Multiplexed Data-Independent Acquisition (mDIA),e a protocol based on multiplexed data-independent acquisition (mDIA) with dimethyl labeling to enhance proteome depth, accuracy, and throughput while minimizing costs. It enables high-quality proteome analysis of single isolated hepatocytes and utilizes liver zonation for single-cell proteomics ben
运动性
发表于 2025-3-26 07:11:01
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Feckless
发表于 2025-3-26 09:14:20
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巫婆
发表于 2025-3-26 13:11:02
Analyzing Posttranslational Modifications in Single Cells,l modifications on some of these proteins. Each new technology that has increased the number of proteins measured per cell has likewise increased our ability to identify and quantify modified peptides. In this chapter, I will discuss our current capabilities, concerns, and challenges specific to thi
阻碍
发表于 2025-3-26 18:05:50
Single-Cell Proteomics Analysis with Tecan Uno and SCREEN Workflow,ogies, it has become increasingly possible to confidently investigate the heterogeneous proteome found within individual cells. In this chapter, we present an automated high-throughput sample preparation workflow based on the Tecan Uno instrument for quantitative single-cell mass spectrometry-based