Jogging 发表于 2025-3-28 14:53:15
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Gernot Hinterleitner,Philipp Hornungropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledgReservation 发表于 2025-3-28 22:54:29
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Gernot Hinterleitner,Philipp Hornunghput and system performance. A unique data analysis system, which in software eliminates the baseline artifact and employs a digital filter to give apparent baseline resolution for each peak in a chromatogram (Hunkapiller, 1987), has improved our ability to obtain sequence information at the low pic方舟 发表于 2025-3-29 10:20:58
http://reply.papertrans.cn/63/6249/624801/624801_45.png可用 发表于 2025-3-29 13:55:54
Gernot Hinterleitner,Philipp Hornunghput and system performance. A unique data analysis system, which in software eliminates the baseline artifact and employs a digital filter to give apparent baseline resolution for each peak in a chromatogram (Hunkapiller, 1987), has improved our ability to obtain sequence information at the low pic一窝小鸟 发表于 2025-3-29 18:48:55
Gernot Hinterleitner,Philipp Hornungoccurring during the transfer. Although employed quite effectively in this application, the DITC glass fiber sheets were mechanically fragile, with rather low protein binding capacity, and it proved difficult to detect the protein molecules on the glass surface.CHOIR 发表于 2025-3-29 20:04:27
Gernot Hinterleitner,Philipp Hornungbundance (less than 1 picomole/mg of purified plasma membrane protein) and their enormous size and complexity. The receptors for insulin and insulin-like growth factor 1 (IGF-1) are both heterotetrameric structures composed of two alpha-subunits of apparent molecular weight 120,000 and two beta-subu蹒跚 发表于 2025-3-30 02:02:02
Gernot Hinterleitner,Philipp Hornungproteins become more complex, e.g., hetero-oligomers, post-translationally modified, larger mass, constraints as to the type and degree of analysis are encountered. This paper describes areas of routine investigation for recombinant proteins and highlights some practical limitations of their analysi飞来飞去真休 发表于 2025-3-30 07:13:58
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