束缚
发表于 2025-3-28 17:24:09
Bioinformatically Informed Design of Synthetic Mammalian Promotersn. Typically, .-regulatory regions from a few genes are randomly combined to generate a synthetic promoter library, and the sequences with the highest activity are selected for in target cell lines. Here we describe a novel approach that can be employed in the construction of synthetic promoters. Sp
Small-Intestine
发表于 2025-3-28 20:33:57
http://reply.papertrans.cn/63/6221/622041/622041_42.png
假
发表于 2025-3-29 02:11:10
Constructing Strong Cell Type-Specific Promoters Through Informed Designtype-specific promoter regulation is a function of each cell’s unique complement of transcriptional machinery components. Accordingly, to achieve high levels of transcriptional activity within a particular cell type, synthetic promoters must be specifically designed to harness those cells discrete r
Temporal-Lobe
发表于 2025-3-29 06:26:21
PCR Assembly of Synthetic Promotersealing between complimentary DNA sequences to build random TFBS combinations within the assembled PCR products. A DNA polymerase is then employed to fill in the unpaired nucleotides in the generated sequences and also to amplify the assembled PCR products. We have used this method to generate synthe
Urologist
发表于 2025-3-29 11:17:30
The Tetracycline Responsive Systemtal in studies examining biological roles of proteins, or continued expression may be unnecessary after therapeutic effects have been achieved in gene therapy. We have utilized pharmacologically regulated gene expression systems to achieve fine control of gene expression levels which facilitate rese
消息灵通
发表于 2025-3-29 12:17:43
Light-Responsive Promotersgulated systems. The latest generation of those light-regulated systems that are specifically responsive to different wavelengths allows spatiotemporal control of gene expression in a so far unprecedented manner..In this chapter, we first describe the available light-inducible gene expression system
极大的痛苦
发表于 2025-3-29 18:45:39
http://reply.papertrans.cn/63/6221/622041/622041_47.png
胶水
发表于 2025-3-29 23:00:46
Promoter Activation with Electromagnetismiation. Promoter activation with extremely low-frequency electromagnetic fields is possible with an appropriate promoter, containing electromagnetic field response elements. Here, we describe how to examine promoter activation with extremely low-frequency electromagnetic fields, and we provide a ste
gout109
发表于 2025-3-30 01:54:26
http://reply.papertrans.cn/63/6221/622041/622041_49.png
angina-pectoris
发表于 2025-3-30 05:47:36
Ryohei Ogawa Ph.D.,Go Kagiya,Akihiko Watanabe,Akihiro Morii,Zheng-Guo Cui,Takashi Kondoss the types of changes in the magnetic and superconducting properties which can occur when a solid is subjected to high pressures. The changes observed for the elemental solids across the periodic table are representative for all compounds and alloys. The only elemental solids which exhibit strong