名词 发表于 2025-3-25 05:43:07
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Single Cell Analysis of , and ,-Infected , by Agarose Embedmentractions are frequently conducted using clonal bacterial populations and population level analysis, overlooking the remarkable differences in single cell behavior. The fastidious nutrient requirements of extracellular . and the extraordinary motility of . hamper an analysis at single cell resolution自传 发表于 2025-3-25 11:40:08
Mitochondrial Dynamics and Activity in ,-Infected Cellsmitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (.) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochon背带 发表于 2025-3-25 16:45:50
Perturbation of , Cell Infection by RNA InterferenceA interference (RNAi) has proven to be a robust and economic technique to study .-host cell interactions. Predesigned and often validated double-stranded (ds) RNA oligonucleotides that silence specific genes are commercially available. RNAi results in a reduced level of distinct proteins, which allo里程碑 发表于 2025-3-25 20:12:42
Isolation of the Dot/Icm Type IV Secretion System Core Complex from e here the procedure to isolate the core complex of the Dot/Icm type IV secretion system of . based on detergent lysis of bacteria and ultracentrifugation. The isolated protein complex can be applied for biochemical and transmission electron microscopy analysis.男生如果明白 发表于 2025-3-26 03:47:23
Carmen Buchrieser,Hubert HilbiIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts提名 发表于 2025-3-26 05:20:49
Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/583814.jpg殖民地 发表于 2025-3-26 09:20:50
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The Pathometabolism of , Studied by Isotopologue Profiling of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living ..Infiltrate 发表于 2025-3-26 16:50:05
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