Deference 发表于 2025-3-25 03:43:44
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Andrea Laghi,Marco Rengoe mitochondria are O.-limited, whereas others suggest that lactate production occurs when O. to the mitochondria is adequate and that the increased lactate production is due to a “mass-action effect” when pyruvate production exceeds the rate of pyruvate oxidation. Pyruvate dehydrogenase is a rate-liHumble 发表于 2025-3-25 16:41:58
Andrea Laghi,Marco Rengoent, including hypoxia. Although the most studied cellular signaling pathway induced by hypoxia is mediated by the transcriptional activity of hypoxia-inducible factors (HIFs), several HIF-independent mechanisms have been implicated in hypoxic adaptation, especially in the regulation of macromoleculCON 发表于 2025-3-25 23:00:24
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http://reply.papertrans.cn/59/5802/580159/580159_27.png分开如此和谐 发表于 2025-3-26 10:00:01
Andrea Laghi,Marco Rengo cell lines cultured in normoxic and hypoxic conditions. We elaborate the methodology of cell culture, hypoxic exposure, metabolite extraction, and relative quantification of polar metabolites from normoxia- and hypoxia-exposed cell extracts, using a liquid chromatography-coupled tandem mass spectroAscendancy 发表于 2025-3-26 16:14:57
http://reply.papertrans.cn/59/5802/580159/580159_29.pngEuphonious 发表于 2025-3-26 18:00:25
Andrea Laghi,Marco Rengoevaluated in two-dimensional (2D) and 3D cultivation platforms during exposure to hypoxia (1% O.) and normoxia (21% O.) using fluorescence microscopy and flow cytometry. We show that hypoxia reporter MSCs exhibit a hypoxia-induced fluorescence signal in both 2D and 3D cultivation platforms with fast