Cardioplegia
发表于 2025-3-26 23:20:59
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蜿蜒而流
发表于 2025-3-27 02:50:05
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poliosis
发表于 2025-3-27 07:35:26
Dissecting Kinase Effector Signaling Using the RapRTAP Methodologyplex in living cells. This technology uses a rapamycin-inducible kinase activation coupled to interaction with FKBP12-binding domain (FRB) tagged protein. Here, we demonstrate application of this method by targeting Src to either p130Cas or FAK and discriminating cell mophodynamic changes downstream each of these signaling complexes.
UNT
发表于 2025-3-27 11:20:57
Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometryan example, we provide an optimized protocol featuring on-bead protein digestion and single nano-flow liquid chromatographic-mass spectrometric (LC-MS) analyses, consequently increasing analytical throughput and sensitivity over gel-based sample preparation methods for rapid profiling of kinase inhibitor targets.
食品室
发表于 2025-3-27 14:56:17
Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Linesust two-stage isolation of phosphotyrosine peptides and mass spectrometry-aided identification of phosphosites to characterize basal signaling networks in unstimulated non-small cell lung cancer (NSCLC) cell lines.
悲观
发表于 2025-3-27 20:25:38
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Calculus
发表于 2025-3-27 23:44:13
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GLIDE
发表于 2025-3-28 04:39:28
Dissecting Kinase Effector Signaling Using the RapRTAP Methodologyponse. Dissecting downstream signaling pathways of kinases is a key step to understanding physiological and pathological cell process. However, directing kinase activity to specific substrates remains challenging. Here, we present a new tool to selectively activate a kinase in a specific protein com
显微镜
发表于 2025-3-28 09:44:42
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daredevil
发表于 2025-3-28 13:50:22
Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors disease phenotypes. When activity data is collected across a panel of kinases, a unique “signaling fingerprint” is generated that allows for discrimination between diseased and normal tissue. Here we describe the use of peptide-based kinase activity sensors to fingerprint the signaling changes asso