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Recombinant Biogenesis and Analysis of Cytochrome , Speciesesenting an avenue for the study of cytochrome . or the cytochrome . biogenesis pathways responsible for heme attachment. Here, we describe a method for recombinant . production of holocytochrome . utilizing the System I (CcmABCDEFGH) bacterial cytochrome . biogenesis pathway, followed by analysis oAGONY 发表于 2025-3-24 17:44:40
Ultra-Performance Liquid Chromatography (UPLC) Analysis of Heme Biosynthesis IntermediatesThe first product, ALA, was derivatized to a highly fluorescent pyrrolizine; PBG, the second intermediate, was enzymatically converted to uroporphyrinogen, and all the porphyrinogen intermediates were oxidized in acid to form fluorescent porphyrins. Heme was measured as hemin. The stable porphyrin f催眠药 发表于 2025-3-24 19:26:40
Heterologous Expression and Purification of Eukaryotic ALA Synthase from al hexahistidine affinity tag followed by a SUMO fusion tag for solubility and ease of purification. The plasmid is introduced into competent . cells, and robust protein expression is induced with IPTG. The ALAS cofactor, pyridoxal 5′-phosphate, is inserted during protein production to yield an actiMETA 发表于 2025-3-25 03:03:40
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