Defense 发表于 2025-3-25 05:43:10
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PKG and their associated proteins. Unfortunately, both PKA and PKG are enriched in the pull downs with both immobilized compounds. Although this proved sufficient to identify novel AKAPs, the lower abundance of PKG has seriously hampered the enrichment and identification of novel GKAPs. Here we pres怒目而视 发表于 2025-3-25 14:06:00
lls using targeted genetically encoded FRET biosensors. Key experimental difficulties include gathering sufficient signal from such a small, photon-limited volume, and the susceptibility of cilia to movement artifacts. Other challenges are associated with the fidelity of sensor targeting and the difMets552 发表于 2025-3-25 19:52:23
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ation advice from the experts.Includes supplementary materia.This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling. The chapters, divided into eight major parts, guide readers through methods on synthSemblance 发表于 2025-3-26 05:17:38
e opportunity to interrogate about the roles of specific matrix components in shaping biofilm architecture. Here, we describe two methods optimized for . that profit from morphology and the high level of structural organization of macrocolonies to gain insight into the production and assembly of amy马笼头 发表于 2025-3-26 09:25:48
e opportunity to interrogate about the roles of specific matrix components in shaping biofilm architecture. Here, we describe two methods optimized for . that profit from morphology and the high level of structural organization of macrocolonies to gain insight into the production and assembly of amyANTH 发表于 2025-3-26 13:53:16
e opportunity to interrogate about the roles of specific matrix components in shaping biofilm architecture. Here, we describe two methods optimized for . that profit from morphology and the high level of structural organization of macrocolonies to gain insight into the production and assembly of amymolest 发表于 2025-3-26 17:49:24
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