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Titlebook: Induced Pluripotent Stem (iPS) Cells; Methods and Protocol Andras Nagy,Kursad Turksen Book 2022Latest edition The Editor(s) (if applicable)

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Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts nerative therapy and drug discoveries. Similar to embryonic stem cells, iPSCs have the capability to differentiate into mature functional iCMCs. The objective of our study is to develop an animal-free and viral-free approach by using a highly efficient transfection method that utilizes a critical co
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Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells,SCs). Here we describe a novel procedure for simultaneous differentiation of hiPSC into muscle cells and motor neurons, that generates innervated and contractile multinucleated skeletal muscle fibers with sarcomeric organization. Our protocol permits the production of expandable skeletal muscle prog
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Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming,imary human neural samples, derivation of proliferative neural progenitor cells (NPCs) from cells harvested by minimally invasive sampling is a key issue. Here we describe a “shortcut” method to establish proliferative NPC cultures directly from peripheral blood mononuclear cells (PBMCs) via interru
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Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenns in MSC capabilities based on their origin, donor age, and culturing method. Recently, human pluripotent stem cells (hPSCs) have been proposed as an alternative renewal source for generating MSCs with large number. Herein, we describe our recently established All-. retinoic acid (RA)-based approac
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The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts,unds to heal for a variety of reasons, including aberrant ECM, dysregulation of vascularization, and persistent inflammation. Tissue engineering approaches, such as porous collagen-based scaffolds, have shown promise in replacing the current treatments of surgical debridement and topical treatments.
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grams has great inertia and low sensitivity..The authors have suggested morphological, temporal, spectral methods of analysis of arterial oscillograms. These methods were used to study the adaptive capacity of cardiovascular system to compression of blood vessels in arm during rest and application o
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