Facilities
发表于 2025-3-30 10:54:58
Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template,study of structure-function relationships or modification of restriction endonuclease restriction sites. Several novel polymerase chain reaction (PCR)-based methods of in vitro mutagenesis have been described (.–.). Site-specific mutations are created by introducing mismatches into the oligonucleoti
VEST
发表于 2025-3-30 14:46:50
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无辜
发表于 2025-3-30 19:05:20
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宽宏大量
发表于 2025-3-30 21:54:53
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花束
发表于 2025-3-31 01:04:44
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Water-Brash
发表于 2025-3-31 08:53:31
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Nonthreatening
发表于 2025-3-31 11:02:54
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CLAM
发表于 2025-3-31 15:47:42
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interpose
发表于 2025-3-31 20:39:37
Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template,so been widely used as a quick and efficient alternative for introducing mutations into specific DNA sequences. Various general methods for site-directed mutagenesis using the PCR have been described recently (.–.). In the overlap extension method two pairs of primers are used to generate two DNA fr
多嘴多舌
发表于 2025-3-31 23:03:12
Site-Directed Mutagenesis In Vitro by Megaprimer PCR,le. The method utilizes three oligonucleotide primers and two rounds of PCR performed on a DNA template containing the cloned gene to be mutated (.–.). The basic megaprimer procedure is shown schematically in ., where A and B represent the “flanking” primers that can map either within the cloned gen