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Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,öhler and Milstem (.) These workers have shown that lymphocytes can be immortalized and subsequently cultured after so1atic fusion with genetically selected myeloma cells. Usually, once fusion between spleen cells and myeloma cells has been performed, cells are suspended in a large volume of selectifluoroscopy 发表于 2025-3-22 17:27:29
Synthesis of Peptides for Use as Immunogens, cells or tissues. With the advent of recombinant DNA technology, it is now often possible to deduce the primary amino acid sequence of a polypeptide without its purification. Two strategies then exist to raise appropriate antibodies. The gene can be expressed in a heterologous species, usually bact火花 发表于 2025-3-22 22:31:27
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IgG Purification,e supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin (Ig) is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity c