NADIR
发表于 2025-3-28 15:42:31
Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen,neous proteins, are often difficult to obtain. The main reasons for this are the small amounts of protein available after the various classical purification processes and the low purity of the proteins.
musicologist
发表于 2025-3-28 20:15:13
http://reply.papertrans.cn/47/4622/462115/462115_42.png
喊叫
发表于 2025-3-29 02:50:24
Production and Characterization of Antibodies Against Synthetic Peptides,he methods described below are designed to give optimal results with minimal injury to the test animal, and we have used them extensively and successfully for several years (.–.). Peptide immunizations differ from those in which the immunogen is a larger macromolecule in that maximal antipeptide tit
Pantry
发表于 2025-3-29 05:19:25
http://reply.papertrans.cn/47/4622/462115/462115_44.png
相互影响
发表于 2025-3-29 10:14:49
Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,öhler and Milstein (.). These workers have shown that lymphocytes can be immortalized and subsequently cultured after somatic fusion with genetically selected myeloma cells. Usually, once fusion between spleen cells and myeloma cells has been performed, cells are suspended in a large volume of selec
COMA
发表于 2025-3-29 11:48:25
Purification of Immunoglobulin G (IgG),re supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity colum
门闩
发表于 2025-3-29 19:12:06
Enzyme-Antienzyme Method for Immunohistochemistry,is labeled with an enzyme that can be reacted with a suitable substrate to give a colored product. The alternative is to use a fluorescent label. The advantage of an enzyme label is that the nuclei can be counterstained, thereby revealing the tissue architecture, and that the stain fades slowly, if
责问
发表于 2025-3-29 22:45:56
http://reply.papertrans.cn/47/4622/462115/462115_48.png
fetter
发表于 2025-3-30 03:49:00
http://reply.papertrans.cn/47/4622/462115/462115_49.png
Abbreviate
发表于 2025-3-30 04:17:44
,Avidin–Biotin Technology, application (for general reviews, . refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for immunoassay. Like-wise, an avidin-enzyme conjugate can be used for immunoblotting, immunoassay, light mi