Basilar-Artery 发表于 2025-3-25 06:54:11
Affinity Chromatography — Old Problems and New Approacheso a wide variety of problems is comparatively recent. As long ago as 1910 there were reports of the purification of amylase by adsorption to insoluble starch (2). Tyrosinase was purified in 1953 using a cellulose matrix bearing the inhibitor, diazodizine (3). In both of these examples the power of pcushion 发表于 2025-3-25 09:51:26
http://reply.papertrans.cn/47/4621/462024/462024_22.pngTonometry 发表于 2025-3-25 14:35:35
http://reply.papertrans.cn/47/4621/462024/462024_23.pngobservatory 发表于 2025-3-25 18:38:59
Non-Specific Binding of Proteins by Substituted Agarosesely charged but unrelated proteins invariably were strongly bound by agarose (Sepharose 4B) substituted with an n-alkylamine or with 4-phenyl-n-butylamine (PBA).. Un-substituted agarose or agarose treated with CNBr, but without subsequent addition of amine, did not bind these proteins. Positively chPTCA635 发表于 2025-3-25 20:04:11
A Solid Phase Radioimmune Assay for Ornithine Transcarbamylase by Yalow and Berson (1960) is based on the ability of unlabeled antigen to compete with antigen labeled with a radioactive isotope bound to antibody. After permitting the system to reach equilibrium antibody bound radioactivity was separated from unbound radioactivity and one or the other determine狼群 发表于 2025-3-26 00:14:51
http://reply.papertrans.cn/47/4621/462024/462024_26.png容易做 发表于 2025-3-26 05:08:08
http://reply.papertrans.cn/47/4621/462024/462024_27.png无瑕疵 发表于 2025-3-26 09:50:55
http://reply.papertrans.cn/47/4621/462024/462024_28.pngattenuate 发表于 2025-3-26 16:13:39
http://reply.papertrans.cn/47/4621/462024/462024_29.png系列 发表于 2025-3-26 20:40:22
Affinity Chromatography of Thymidylate Synthetases Using 5-Fluoro-2′-Deoxyuridine 5′-Phosphate Derivthylenetetrahydrofolate donates its labile one-carbon unit (the methylene group) to deoxyuridylate, and also provides the reducing power necessary to convert the methylene group to methyl leading to the formation of thymidylate and dihydrofolate, as is shown in equation (1). The dihydrofolate so for