satisficer 发表于 2025-3-21 19:59:44
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Raghav Rajagopalanrate tissue-specific cell lineage reporters, overexpress genes from a defined locus, and introduce and repair single point mutations in hPSCs. This ability to genetically engineer pluripotent stem cells will significantly facilitate the study of human disease in a defined genetic context. Here we ou帽子 发表于 2025-3-22 11:02:33
Raghav Rajagopalanreby generating Ty5 elements with new target specificities. The mechanism of Ty5 target site choice suggests that integration specificity of other retrotransposons and retroviruses can be altered by engineering integrases to recognize DNA-bound protein partners. Retroelements can also be used to proHeresy 发表于 2025-3-22 12:55:21
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Raghav Rajagopalanes a simple antibiotic selection to isolate cell lines in which the introduced plasmid has integrated at the desired . site. A polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on ϕC31 integrase suppliesSmall-Intestine 发表于 2025-3-23 02:20:05
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