Harness 发表于 2025-3-23 10:45:39
Derek Pardue of [.S]-labeled purified actin. We include a protocol to purify [.S]-labeled actin overexpressed in bacterial cells. This protocol can be used to purify any other labeled protein following overexpression in bacteria, including luciferase and tubulin. We also describe a protocol to purify [.S]-label有罪 发表于 2025-3-23 17:06:24
Derek Parduee of Ethics of the NSPE, and to the laws of the state of registration. Having presented these qualities the licensee is certified as an Intern Engineer, and the state involved has fulfilled its constitutionally defined responsibility to protect the public.978-0-412-14961-0978-1-4684-8355-0Comprise 发表于 2025-3-23 18:05:43
e of Ethics of the NSPE, and to the laws of the state of registration. Having presented these qualities the licensee is certified as an Intern Engineer, and the state involved has fulfilled its constitutionally defined responsibility to protect the public.978-0-412-14961-0978-1-4684-8355-0良心 发表于 2025-3-24 01:21:16
Introduction and Frame,performers over the past few years have become increasingly nostalgic and yearn to revisit the old days of raw performance and neighborhood battles. Such reflection is evident in the lyrics quoted above. Thaíde, one of the most widely recognized rappers and pioneers of hip hop in Brazil, created a c最低点 发表于 2025-3-24 06:17:58
Assembling Brazilian Hip Hop Histories,iphery” (a popular phrase and the name of popular book ) are reaching a group of interested middle-class students. Presentations and discussions about hip hop as culture for the first time are legitimate debates. These histories focus on hip hop skills, genresmitten 发表于 2025-3-24 08:41:03
Making Territorial Claims,p community, has always prided himself on his collection of music, literature, and other material related to . He refers to himself as a “researcher” and “archivist,” and it was in this spirit that we scheduled my visit.高谈阔论 发表于 2025-3-24 10:40:52
Putting , to Music,he working class in the . In these final two substantive chapters, I discuss how São Paulo hip hoppers do ideological work and strive to articulate dynamic notions of blackness and gender (masculinities and femininities). Again, hip hop is the vehicle of choice (and, at times, necessity) for thousanParley 发表于 2025-3-24 15:14:44
http://reply.papertrans.cn/47/4611/461046/461046_18.pnggraphy 发表于 2025-3-24 20:48:57
, (I’m Out /The End?), of cultural practice as “design” based on a set of ideologies to explain and narrate Brazilian hip hop. Such a keyword is heuristic; it is the spirit behind the ubiquitous hip hop phrase . (“we’s on tape”), central to the opening staement of this ethnography. The ideologies of “we” and representatiMaximizer 发表于 2025-3-25 02:01:23
ts (HSEs). There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy (Wu, Annu Rev Cell Dev Biol 11:441–469, 1995; Morimoto, Genes Dev 12:3788–3796, 1998; Tessari et al., Mol Hum Repord 4:253–258, 2004; Fujimoto et al., Mol Biol Cell 21:106–116, 2010; Nakai et al., Mol Cell B