调整校对 发表于 2025-3-25 04:15:34
David A. Blakessful .Methods in Molecular Biology.™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cutLimited 发表于 2025-3-25 10:31:48
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Leslie Z. Benetid protein expression to the site of virion assembly in mature keratinocytes. Here, I describe the methods in details of how to establish primary mouse keratinocyte culture, to prepare cell-free lysate, to carry out in vitro translation of the viral gene mRNAs, and to detect the translated productsAntagonism 发表于 2025-3-25 19:48:04
Ralph F. Shangrawnsmembrane transport limitations, the need to maintain cell activity, and a long time to assay . Another problem is that cell-based biosensors make use of living, genetically modified microorganisms (GMOs). The application of these GMO biosensors is often not possible due to biosafety concerns, a摘要 发表于 2025-3-25 22:43:23
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http://reply.papertrans.cn/47/4608/460751/460751_26.pngScleroderma 发表于 2025-3-26 05:05:57
http://reply.papertrans.cn/47/4608/460751/460751_27.pngChronic 发表于 2025-3-26 11:54:19
Lisa D. Rarickon (Kozak 1983), and the effects of stress conditions on translation (Morley et al. 1985). Rabbit reticulocyte lysates (RRL) were optimized to produce a highly efficient system and became widely used (Pehlam and Jackson 1976; Jackson and Hunt 1983). However, this system showed properties rarely obse轻快带来危险 发表于 2025-3-26 12:49:43
Hanni Levi Ellison (Kozak 1983), and the effects of stress conditions on translation (Morley et al. 1985). Rabbit reticulocyte lysates (RRL) were optimized to produce a highly efficient system and became widely used (Pehlam and Jackson 1976; Jackson and Hunt 1983). However, this system showed properties rarely obse革新 发表于 2025-3-26 19:08:52
Lillian L. Yintor mimics operate to regulate gene expression inside living cells in a transgene-free and cost-effective manner. Hairpin pyrrole–imidazole polyamides (PIPs) are selective DNA-binding small molecules that can be preprogrammed to read specific DNA sequences. PIPs and their conjugates have been develo