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Joe P. Buhlern milligram amounts in the RTS 500. HY. The non-native proteins were used for immunization and as protein standards for quantitation of the respective proteins in biological material by semi-quantitative Western-blotting. Along the way we also learned some interesting details about the rapid transla业余爱好者 发表于 2025-3-22 00:33:49
Joe P. Buhleressary regulatory elements in the 5’ and 3’ untranslated regions via polymerase chain reaction (PCR). The obtained PCR products could be used directly as templates first for eukaryotic cell-free protein synthesis . Templates designed for use in the more productive prokaryotic proteiIndicative 发表于 2025-3-22 05:17:19
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Joe P. Buhler canonical amino acid attached on cognate tRNA can be chemically or enzymatically modified or even loaded onto desired tRNA before entering the ribosome . Second, the amino acid selection process in living cells could be affected by experimentally imposed selective pressure-a selective pressure iovershadow 发表于 2025-3-22 16:26:38
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Joe P. Buhlerssful .Methods in Molecular Biology.™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cut套索 发表于 2025-3-22 21:57:13
Joe P. Buhlerssful .Methods in Molecular Biology.™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cutoncologist 发表于 2025-3-23 01:26:40
their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cut978-1-4939-5698-2978-1-60327-331-2Series ISSN 1064-3745 Series E-ISSN 1940-6029LURE 发表于 2025-3-23 05:38:07
978-3-540-08844-8Springer-Verlag Berlin Heidelberg 1978