WATER
发表于 2025-3-25 05:36:32
Reese M. Heitnerchemical variability that could be potentially advantageous for functional diversification of proteins, especially in a user-defined environment. Recent examples of such artificial (i.e., tailor-made) proteins include functional design of novel classes of protein pH sensors, novel classes of green f
OVER
发表于 2025-3-25 08:27:26
the rapid translation system (RTS), an .-based cell-free system. The proteins selected for this feasibility study represent four classes of membrane proteins that are found in organelles, involved in endo-and exocytosis (Fig. 16.1A) (reviewed in High and Laird 1997), i.e., a type-I membrane protein
发牢骚
发表于 2025-3-25 14:37:36
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Bmd955
发表于 2025-3-25 16:45:24
cal, .Cell‐Free Gene Expression: Methods and Protocols. serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications..978-1-0716-2000-7978-1-0716-1998-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
债务
发表于 2025-3-25 21:35:39
Reese M. Heitner gradient. This method also includes a protocol to pause and restart translation of membrane proteins at specified positions during their co-translational folding. This stop–start strategy provides an avenue to investigate whether the proteins fold in sequence order, or if the correct fold of N-term
小平面
发表于 2025-3-26 03:12:27
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agonist
发表于 2025-3-26 04:22:12
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无弹性
发表于 2025-3-26 12:18:28
n milligram amounts in the RTS 500. HY. The non-native proteins were used for immunization and as protein standards for quantitation of the respective proteins in biological material by semi-quantitative Western-blotting. Along the way we also learned some interesting details about the rapid transla
高深莫测
发表于 2025-3-26 16:24:04
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accrete
发表于 2025-3-26 17:49:55
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