毁坏 发表于 2025-3-28 17:53:57

,Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture,cycle. In this chapter, we describe in detail how to (1) culture keratinocytes, (2) electroporate primary keratinocytes with HPV DNA, (3) detect episomal HPV genomes by Southern (DNA) blotting, (4) grow organotypic raft cultures, (5) isolate HPV, and (6) perform in vitro infectivity testing.

直言不讳 发表于 2025-3-28 20:05:35

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Senescent 发表于 2025-3-29 00:46:19

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ingestion 发表于 2025-3-29 04:57:23

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字形刻痕 发表于 2025-3-29 08:15:08

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无弹性 发表于 2025-3-29 11:24:28

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LAVE 发表于 2025-3-29 16:50:15

Book 2006study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze pa

甜得发腻 发表于 2025-3-29 23:13:46

Identification of New Papillomavirus Types,f these infections to a multiplicity of clinical manifestations. The cloning and sequencing of PCR-amplified products has, to date, resulted in the identification of more than 300 putative “new” papillomavirus types. The methods used to identify these unknown papillomavirus sequences are described h

micronutrients 发表于 2025-3-30 00:25:00

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注意到 发表于 2025-3-30 06:12:44

Histochemical Analysis of Cutaneous HPV-Associated Lesions,ous human papillomavirus-associated lesions. Every step of the staining procedure, from sampling of the specimens to microscopic examination of the stained sections, is detailed with reference to the recent achievements in this field.
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查看完整版本: Titlebook: Human Papillomaviruses; Methods and Protocol Clare Davy,John Doorbar Book 2006 Humana Press 2006 DNA.PCR.Southern Blot.Virus.cell lines.gen