Harbor
发表于 2025-3-28 16:43:15
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Highbrow
发表于 2025-3-28 20:07:17
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echnic
发表于 2025-3-29 00:22:49
Holliday Junction Resolution,ranslational modifications like phosphorylation. Here, we describe a protocol to purify . Yen1 resolvase in two different phosphorylation states (high and low) and to set up a biochemical assay to compare their ability to process a synthetic, oligonucleotide-based Holliday junction structures.
Dictation
发表于 2025-3-29 06:04:38
Genome-Wide Analysis of Mitotic Recombination in Budding Yeast, with limited experience in computational methods to conceptually understand and execute the steps of genome-wide LOH analysis as well as to adapt and apply them to their own specific studies and experimental models.
呼吸
发表于 2025-3-29 10:00:35
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含糊其辞
发表于 2025-3-29 13:08:11
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interrogate
发表于 2025-3-29 17:27:46
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HALO
发表于 2025-3-29 21:47:52
Analysis of DNA Double-Strand Break End Resection and Single-Strand Annealing in ,dditional assay to follow resection involves recombination between two direct repeats by single-strand annealing (SSA), since SSA requires extensive resection to expose two single-strand repeats for annealing. The kinetics of resection and SSA repair are then measured using Southern blots.
forthy
发表于 2025-3-30 02:31:17
Biochemical Analysis of D-Loop Extension and DNA Strand Displacement Synthesis,s biochemical assays for (1) D-loop formation and DNA synthesis within the D-loop and (2) DNA strand displacement synthesis to test the role of DNA helicases (e.g., Pif1) in repair DNA synthesis. These mechanistic assays are valuable for elucidating the molecular details of HR.
无目标
发表于 2025-3-30 07:11:59
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