Canopy
发表于 2025-3-23 12:33:57
Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Compl for purification of native chromatin from yeast cells (Chapter .). Here, we present the methods to obtain highly purified native chromatin modifying complexes from ., based on Tandem Affinity Purification (TAP). We also present possible applications and useful functional assays that can be performed using these yeast native reagents.
ALIAS
发表于 2025-3-23 17:03:27
Production and Purification of Antibodies Against Histone Modifications,ions of canonical histones. Our protocols can be applied to obtain antibodies that occur in histone variants and proteins other than histones. In addition, some of our protocols are compatible with the production of monoclonal or recombinant antibodies.
angina-pectoris
发表于 2025-3-23 18:52:18
A Method for Large-Scale Screening of Random Sequence Libraries to Determine the Function of Unstruhod typically identifies sequences that, while being totally different from the wild type, retain essential features allowing yeast to live. This collection of proteins with functional synthetic sequences can subsequently be used in phenotypic tests or genetic screens in order to discover genetic interaction.
光滑
发表于 2025-3-23 23:58:28
http://reply.papertrans.cn/43/4273/427294/427294_14.png
promote
发表于 2025-3-24 04:47:59
Histone Purification from ,,osttranslational modifications that regulate nucleosome interactions, position, and stability..We describe a protocol allowing the robust purification of histones from the yeast .. This method appears to be suitable to quantitatively analyze specific posttranslational histone modifications.
注入
发表于 2025-3-24 08:35:48
Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast,ing sites in vivo. It has been a particularly powerful technique for mapping of histone modification patterns both at individual loci and genome-wide. Here we provide a detailed protocol for ChIP of histone modifications associated with active transcription in fission yeast (.).
发出眩目光芒
发表于 2025-3-24 11:46:28
http://reply.papertrans.cn/43/4273/427294/427294_17.png
喃喃诉苦
发表于 2025-3-24 16:22:19
http://reply.papertrans.cn/43/4273/427294/427294_18.png
潜移默化
发表于 2025-3-24 20:55:00
http://reply.papertrans.cn/43/4273/427294/427294_19.png
ethnology
发表于 2025-3-25 01:22:17
978-1-4939-8254-7Springer Science+Business Media New York 2017