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Application of Fluorescence Polarization in HTS Assays,r interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.garrulous 发表于 2025-3-22 15:27:13
Design and Implementation of High-Throughput Screening Assays,ists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of lead compounds. At every step in this process, there are decisions to be made that can greatly imAccrue 发表于 2025-3-22 18:15:32
Creation of a Small High-Throughput Screening Facility,d organizational effort. Major issues to consider include the selection of equipment, the establishment of data analysis methodologies, and the formation of a group having the necessary competencies. If done properly, it is possible to build a screening system in incremental steps, adding new pieces持续 发表于 2025-3-22 23:55:16
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Enzyme Assay Design for High-Throughput Screening, initial active chemical compounds. The development of fluorescent- or absorbance-based readouts typically remains the formats of choice for enzyme screens and a wealth of experience from both industry and academia has led to a comprehensive set of standardized assay development and validation guide