不可磨灭
发表于 2025-3-27 00:02:58
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Keratin
发表于 2025-3-27 05:05:40
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TOXIN
发表于 2025-3-27 07:51:26
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迫击炮
发表于 2025-3-27 12:51:13
Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expretions and assembled by a single overlap-extension PCR reaction. The amplified product is then cloned into a mammalian expression vector suitable for high-titer transient gene expression. This workflow can be applied to any antibody sequence by adapting the specific primer sequences to the antibody of choice.
薄荷醇
发表于 2025-3-27 17:14:31
Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture,e stability of the recombinant CHO cells over long-term culture. These procedures include the following; western blotting, ELISA to evaluate protein production, real-time PCR to analyze plasmid and mRNA copy numbers, and fluorescent in situ hybridization (FISH) to assess the location of the inserted plasmid on host cell chromosomes.
BYRE
发表于 2025-3-27 19:20:06
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6Applepolish
发表于 2025-3-27 23:28:08
Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension,incubation at 31 °C with agitation by orbital shaking. We also describe an alternative method in which 90% of the pDNA is replaced by nonspecific (filler) DNA, and the production phase is performed at 31 °C in the presence of 0.25% N, N-dimethylacetamide (DMA).
travail
发表于 2025-3-28 05:33:38
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歌剧等
发表于 2025-3-28 09:03:02
Selection of High-Producing Clones Using FACS for CHO Cell Line Development,powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.
inquisitive
发表于 2025-3-28 13:04:52
Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses, while estimation of HCP clearance/presence can be achieved by comparing 2D-PAGE images of samples and by undertaking western blotting of 2D-PAGE analyzed samples. Here, we describe the analyses of HCP content using these methodologies.