拱墙
发表于 2025-3-26 21:18:59
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GUILT
发表于 2025-3-27 02:36:41
Periplasmic Chaperones Used to Enhance Functional Secretion of Proteins in ,,tein aggregation, i.e., formation of inclusion bodies, are frequently encountered. This is particularly true for proteins that carry structural disulfide bonds, including antibody fragments, cytokines, growth factors, and extracellular fragments of eukaryotic cell surface receptors. In these cases,
Conjuction
发表于 2025-3-27 06:29:18
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Bronchial-Tubes
发表于 2025-3-27 10:44:07
The Targeted Expression of Nucleotide Sugar Transporters to the , Inner Membrane,ip between their structure and function. We have therefore utilised the OmpA signal sequence to deliberately target the expression of a mammalian nucleotide sugar transporter, the murine CMP-sialic acid transporter, to the . inner membrane. The functionality of the recombinant CMP-sialic acid transp
mettlesome
发表于 2025-3-27 16:27:23
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PIZZA
发表于 2025-3-27 18:58:17
Enhancing the Solubility of Recombinant Proteins in , by Using Hexahistidine-Tagged Maltose-Bindingbinding protein (MBP) has been recognized as one of the most effective solubilizing agents, having frequently been observed to improve the yield, enhance the solubility, and promote the proper folding of its fusion partners. The use of a dual hexahistidine–maltose-binding protein affinity tag (His.–
Gustatory
发表于 2025-3-27 23:13:41
Introducing Predetermined Mutations Throughout a Target Gene Using TDEM (Transposon-Directed Base-ETDEM removes a predetermined number of bases (up to 11 base pairs) from a randomly selected site within the target gene and replaces them with any length of DNA of predetermined sequence. Therefore, the number of bases to be deleted and inserted can be precisely regulated. Because each round of TDEM
Infiltrate
发表于 2025-3-28 02:42:55
Fluorescent Site-Specific Labeling of , Expressed Proteins with Sfp Phosphopantetheinyl Transferaseo-tag system for expression and isolation of a protein of interest from . and subsequent site-specific fluorescent labeling with Sfp phosphopantetheinyl transferase (Sfp synthase). In the example presented, adenoviral protein E3-14.7 K (E14.7) was expressed as a tripartite fusion protein with a fluo
OVERT
发表于 2025-3-28 10:06:33
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挫败
发表于 2025-3-28 13:21:45
Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins, correctly even in optimized culture conditions. In this case, protein aggregates can be recovered and their refolding assisted by an osmolyte/chaperone-dependent system. The selection of aggregates with different degrees of complexity can be exploited to maximize the yields of native proteins at the end of the refolding process.