vasculitis 发表于 2025-3-30 11:24:42
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Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simpless introduction of any mutation using modern tools of bacterial genetics. To clone a herpes simplex virus genome, a BAC replication origin is first introduced into the viral genome by homologous recombination in eukaryotic host cells. As part of their nuclear replication cycle, genomes of herpesvi材料等 发表于 2025-3-30 20:05:47
Engineering HSV-1 Vectors for Gene Therapy,ybera (alipogene tiparvovec) as the first gene therapy product as a standard medical treatment (Yla-Herttuala, Mol Ther 20: 1831–1832, 2013), gene therapy has reached the status of being a part of standard patient care. Replication-competent herpes simplex virus (HSV) vectors that replicate specificMELD 发表于 2025-3-30 21:51:21
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Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactiveactivation. While several alternative models are available, including infections of live animal and explanted ganglia, these are complicated by the presence of multiple cell types, including immune cells, and difficulties in manipulating the neuronal environment. The highly pure neuron culture systGENUS 发表于 2025-4-1 00:10:34
Characterization of Extracellular HSV-1 Virions by Proteomics,avor the detection of true components. The protocol described below involves the removal of highly contaminating serum proteins and purification of the virions by a series of differential and density centrifugation steps. In addition, L-particles, which are viral particles devoid of genome and capsi