召集 发表于 2025-3-25 05:21:05
Full-Length Open Reading Frame Amplification of Hepatitis C Virusreaction (RT-PCR) approach. Our method relies on a highly robust and sensitive RNA extraction procedure and cutting-edge RT-PCR enzymes, all of which have been rigorously tested and optimized. This will not only allow for robust amplification of the entire open reading frame (ORF) of HCV for sequencFAWN 发表于 2025-3-25 08:09:40
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Molecular Identification of Transmitted/Founder Hepatitis C Viruses and Their Progeny by Single Genospecies complexity can vary substantially during the course of natural infection as a consequence of viral population “bottlenecking.” This occurs at the time of transmission from one individual to the next and during the course of chronic infection of an individual when adaptive immune responses elPhagocytes 发表于 2025-3-26 00:13:59
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Expression Cloning of Host Factors Required for the HCV Replication Cycleends of a number of factors encoded by its host cell to execute its replication cycle and create. Expression cloning is one of several possible approaches that have been employed to identify novel host factors essential for the HCV replication cycle. It involves generation of a cDNA library from a cItinerant 发表于 2025-3-26 08:36:36
Application of Deep Mutational Scanning in Hepatitis C Virustness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.enhance 发表于 2025-3-26 15:11:26
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