Titlebook: Hepatitis C Protocols; Johnson Yiu-Nam Lau Book 1998 Springer Science+Business Media New York 1998 infectious diseases

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Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridizationections (.–.). The assay can detect HCV in HCV antibody-negative individuals suspected of having hepatitis and can discriminate chronic HCV infections from resolved acute infections in patients who are positive for HCV antibody. The procedure can also be used to:

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Serological Genotyping Using Synthetic Peptides Derived from the NS4 Regionapy when compared to genotypes 2 or 3 (reviewed in ..,.) This discovery emphasizes the need for genotyping methods in current clinical practice, in providing a predictor of the outcome of treatment, and perhaps helping to target appropriate treatment to the most relevant patient groups.

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Determination of HCV Quasispecies by Cloning and Sequencingons he within the envelope 2 region, which are referred to as hypervariable regions (HVRs). The HVRs in HCV are believed to be the result of high nucleotide variation and immune selection pressure (.). Because of its heterogeneity, this is a good region for the study of HCV quasispecies.

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Book 1998 de force of modern molecular medicine. This breakthrough not only unearthed the causative agent of non-A non-B hepatitis that had eluded the best of scientists for more than 20 years, but also was symbolic of another chapter in the changing paradigm of modern science and medicine. The re- lutionary

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Quality Control of the Polymerase Chain Reaction, mRNA analysis (.), DNA and RNA quantitation (.–.), mutagenesis (.), and gene detection (.). Because of its ability to amplify minute quantities of nucleic acid specifically, PCR has also been applied with great success in clinical diagnostics (.–.), genetic testing (.–.), forensics (.,.), and environmental testing (.,.).

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Titlebook: Hepatitis C Protocols; Johnson Yiu-Nam Lau Book 1998 Springer Science+Business Media New York 1998 infectious diseases

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