富饶
发表于 2025-3-27 00:59:31
,Investigation of Protein–Protein Interactions and Conformational Changes in Hedgehog Signaling Pathdern life sciences. Among various techniques developed to study such phenomena, fluorescence resonance energy transfer (FRET) is a widely used method with many advantages in detecting these molecular events. Here, we describe the application of FRET in the mechanistic investigation of cell signal tr
禁令
发表于 2025-3-27 03:07:01
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Awning
发表于 2025-3-27 07:20:23
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旋转一周
发表于 2025-3-27 13:07:58
Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components,elative stoichiometries and affinities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and
碎石头
发表于 2025-3-27 13:55:16
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colostrum
发表于 2025-3-27 20:47:18
Rapid Screening of Gli2/3 Mutants Using the Flp-In System, proteins has been hampered by the fact that their overexpression in cells prevents their proper regulation. To address this issue, we have developed a method of rapid generation of stable cell lines expressing near-endogenous and approximately equal levels of wild-type and mutant Gli proteins. This
allude
发表于 2025-3-28 01:04:01
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brother
发表于 2025-3-28 03:40:39
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pantomime
发表于 2025-3-28 06:38:52
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Exuberance
发表于 2025-3-28 12:51:19
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