揉杂 发表于 2025-3-26 22:06:13
Book 2018book discuss high-throughput methods for HLA typing; wet lab protocols; microarray data and its uses; in silico tools for the identification of HLA alleles from DNA and RNA next-generation-sequencing data, as well as HLA haplotype frequency estimation. Written in the highly successful .Methods in Mo缓解 发表于 2025-3-27 03:55:56
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,High-Throughput Contiguous Full-Length Next-Generation Sequencing of HLA Class I and II Genes from d recent advances in next-generation sequencing (NGS) technologies have facilitated the development of whole gene sequencing methods. We describe an accurate, efficient, scalable, and cost-effective approach to contiguously amplify and sequence full-length genes of six HLA class I and II loci from 96 individuals on a single Illumina MiSeq run.松紧带 发表于 2025-3-27 10:03:46
Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA,mens are considered a noninvasive and cost-effective method for registry typing. Here, we describe the feasibility of using long-range PCR and clonal sequencing by Illumina MiSeq to deliver unambiguous HLA typing on buccal-based donor recruitment samples.objection 发表于 2025-3-27 16:22:08
In Silico Typing of Classical and Non-classical HLA Alleles from Standard RNA-Seq Reads,eq2HLA, that takes standard RNA-Seq reads as input and determines a sample’s (classical and non-classical) HLA class I and class II types as well as HLA expression. We demonstrate the application of seq2HLA using publicly available RNA-Seq data from the Burkitt’s lymphoma cell line DAUDI and the choriocarcinoma cell line JEG-3.Estrogen 发表于 2025-3-27 21:42:54
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