胶水
发表于 2025-3-25 03:46:56
Single-Turnover RING/U-Box E3-Mediated Lysine Discharge AssaysRING and U-box ubiquitin ligases promote ubiquitin (Ub) transfer by priming Ub-conjugated E2 in a closed conformation to optimize the thioester bond for nucleophilic attack by substrate lysine. Here, we describe a single-turnover lysine discharge assay for direct assessment of the activity of any RING/U-box E3-E2~Ub complex.
昏暗
发表于 2025-3-25 10:28:57
http://reply.papertrans.cn/40/3916/391513/391513_22.png
Chromatic
发表于 2025-3-25 12:36:52
http://reply.papertrans.cn/40/3916/391513/391513_23.png
insightful
发表于 2025-3-25 15:56:40
A Blueprint for ReformWith the end of the cold war, the role of the United Nations in peacemaking and peace-keeping has increased, but in the social and economic sphere it has been reduced. No longer is an arbiter above the cold-war battleground needed, and many of its functions have been handed over to the World Bank, to NGOs and to bilateral donors.
躲债
发表于 2025-3-25 21:45:53
http://reply.papertrans.cn/40/3916/391513/391513_25.png
VOK
发表于 2025-3-26 02:21:40
http://reply.papertrans.cn/40/3916/391513/391513_26.png
声明
发表于 2025-3-26 08:09:38
Profile of the organizations who have promoted benchmarkingThis section gives a profile of the organizations within the UK who are active in the field of benchmarking. The profile reviews the organization, its methodology and relevant work or publications.
可卡
发表于 2025-3-26 08:32:03
http://reply.papertrans.cn/40/3916/391513/391513_28.png
miracle
发表于 2025-3-26 16:07:51
Grab ‘Em by the Legacy: Rolling Back the Years with President TrumpThis chapter will consider what the Trump presidency means for women, in terms of style and substance. Some consideration will be given to Trump’s tone and rhetoric both on the campaign trail and as president, along with his stated policy priorities.
fulmination
发表于 2025-3-26 17:52:04
How to avoid pitfallsThere are currently more than a dozen benchmarking processes in use by various companies. They all have a similar framework and contain the same basic elements of: