变成小松鼠 发表于 2025-3-21 18:02:25

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Occupation 发表于 2025-3-21 22:41:44

Separation and Identification of Mucins and Their Glycoformsse mucins share common features in that they are oligomeric in nature and consist of a variable number of monomers (subunits) linked in an end-to-end fashion via the agency of disulfide bonds. In addition, their polypeptides comprise regions of dense glycosylation interspersed with “naked” cysteine-rich domains (.–.).

Champion 发表于 2025-3-22 01:10:02

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翻动 发表于 2025-3-22 06:02:31

Identification of Glycosylation Sites in Mucin Peptides by Edman Degradationicroheterogeneity). Typically, the characterization of macro- and microheterogeneity has been dependent on the isolation of small peptides with only one glycosylation site. However, this is not possible with high molecular weight, heavily glycosylated domains such as those found in mucins.

过份艳丽 发表于 2025-3-22 11:01:20

Mucin Domains to Explore Disulfide-Dependent Dimer Formationregion of tandem repeat sequences, flanked by cysteine-rich regions at each end, which are presumed to mediate polymerization. Secretory mucins contain approx 60–80% carbohydrate, with extensive .-glycosylation in the central tandem repeat regions, and N-linked oligosaccharides in the peripheral regions (.).

水土 发表于 2025-3-22 14:36:20

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水土 发表于 2025-3-22 20:43:19

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龙卷风 发表于 2025-3-22 22:45:26

Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods starting point for development of a technique. Refer to Chapter 3 for detection of mucins in histological preparations (.); note, however, that many of the principles for selection of detection tools discussed herein are applicable to histological detection.

钱财 发表于 2025-3-23 02:58:01

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blister 发表于 2025-3-23 08:38:19

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查看完整版本: Titlebook: Glycoprotein Methods and Protocols; The Mucins Anthony P. Corfield Book 2000 Humana Press 2000