ineffectual
发表于 2025-3-28 14:45:47
Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF,ype. The variability in blood perfusion and oxygen tension within tumors suggests that ambient pH values fluctuate across different tumor territories. This chapter describes a detailed protocol for measuring intracellular pH in patient-derived GBM cells in vitro, using the fluorescent pH sensitive d
光明正大
发表于 2025-3-28 21:49:52
Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro,cussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.
不自然
发表于 2025-3-29 02:31:20
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NEXUS
发表于 2025-3-29 06:45:26
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规章
发表于 2025-3-29 09:41:41
Single-Cell RNA Sequencing of Glioblastoma Cells,ional RNASeq in which entire populations are sequenced in bulk, sc-RNASeq can be beneficial when trying to better understand gene expression patterns in markedly heterogeneous populations of cells or when trying to identify transcriptional signatures of rare cells that may be underrepresented when u
传染
发表于 2025-3-29 15:02:50
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ELUDE
发表于 2025-3-29 15:52:14
Orthotopic Patient-Derived Glioblastoma Xenografts in Mice,se brain are obtained by injection of GBM stem-like cells derived from fresh surgical specimens. These xenografts reproduce GBM’s histologic complexity and hallmark biological behaviors, such as brain invasion, angiogenesis, and resistance to therapy. This method has become essential for analyzing m
使饥饿
发表于 2025-3-29 23:17:58
Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice,noninvasive imaging. This chapter describes in detail how to assess xenograft size and growth using bioluminescent imaging with IVIS (in vivo imaging system). This form of imaging (a) can be performed without the help of a trained technician, (b) is a very quick procedure, allowing simultaneous imag
详细目录
发表于 2025-3-30 03:23:56
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Asymptomatic
发表于 2025-3-30 04:12:40
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