同音 发表于 2025-3-23 20:56:20
Das Haager Übereinkommen von 2005stem which make this possible include the following: the availability of selectable markers; the ease of transformation of yeast with exogenous DNA; the integration of DNA into yeast chromosomes by homologous recombination; and the ease of genetic manipulation of yeast cells. As an illustration of sGranular 发表于 2025-3-23 22:12:34
http://reply.papertrans.cn/39/3826/382515/382515_14.png抱狗不敢前 发表于 2025-3-24 04:39:32
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http://reply.papertrans.cn/39/3826/382515/382515_16.pngarboretum 发表于 2025-3-24 12:43:43
https://doi.org/10.1007/978-3-7091-6398-6romosomes. involves phagocytosis and there is a requirement for co-precipitation of the donor DNA. or chromosomes. with calcium phosphate to achieve optimal frequencies of gene transfer. In either method, transformants usually initially exhibit an unstable phenotype, and stabilization ultimately occRotator-Cuff 发表于 2025-3-24 16:13:03
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https://doi.org/10.1007/978-3-662-39798-5ent (Miller, 1981a). Among the features which make baculoviruses highly advantageous as recombinant DNA vector systems are (1) a covalently-closed, circular, nuclear-replicating DNA genome, (2) an extendable rod-shaped capsid, (3) a group of genes, involved in occlusion, that are nonessential for in潜移默化 发表于 2025-3-25 05:34:37
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https://doi.org/10.1007/978-3-642-60225-2can be efficiently transferred into a wide variety of Gram-negative bacteria by a separate mobilizing plasmid. One of the vectors is a cosmid and can be used for cloning large DNA fragments. The vectors, which can be selected on the basis of kanamycin, spectinomycin, or chloramphenicol resistance, c