勤劳
发表于 2025-3-25 03:27:55
Choice and Manipulation of Retroviral Vectors,in tissue-culture experiments. In comparison, chemical and electrical means of gene transfer require large initial numbers of cells and, under optimal circumstances, function at efficiencies several orders of magnitude lower than retroviral vectors, and only in a limited number of specific cell types.
Negligible
发表于 2025-3-25 10:48:57
Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell L wild-type cell line, one had first to select a recessive mutant derivative cell line and characterize it before proceeding with the experiments of interest. Such restrictions posed a significant barrier to molecular genetic analyses in higher eukaryotic cells.
幼稚
发表于 2025-3-25 12:57:54
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带伤害
发表于 2025-3-25 19:38:28
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Bravura
发表于 2025-3-25 22:17:08
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LITHE
发表于 2025-3-26 01:21:30
Physics and Technology of SPECT/CT,s or cells in culture, and analyze the levels of gene product produced by the introduced construct. Ideally, such a reporter gene should encode a product that is stable, innocuous to the cell or organism in which it is being expressed, and should be readily detectable, even when present in small quantities.
Lymphocyte
发表于 2025-3-26 07:32:22
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Alcove
发表于 2025-3-26 09:14:46
Manipulation of Baculovirus Vectors,w to the field. However, before describing the methodology peculiar to baculovirus expression vectors, it is of value to briefly consider the isolation, structure, and replication strategy of this unique group of viruses. Detailed and more comprehensive reviews that describe the biology of baculoviruses are available (.–.)
外面
发表于 2025-3-26 13:19:54
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Explicate
发表于 2025-3-26 17:44:13
,Use of , (β-Galactosidase) as a Reporter Gene,s or cells in culture, and analyze the levels of gene product produced by the introduced construct. Ideally, such a reporter gene should encode a product that is stable, innocuous to the cell or organism in which it is being expressed, and should be readily detectable, even when present in small quantities.