Titlebook: Gene Therapy Protocols; Volume 2: Design and Joseph M. Doux Book 20083rd edition Humana Press 2008 DNA.Promoter.Virus.gene expression.gene

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Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cell first-generation viral transduction systems were engineered for constitutive expression of a single therapeutic transgene, most advanced viral gene-transfer technologies enable regulated expression of several transgenes. Efficient transfer of numerous transgenes enables co-expression of therapeutic

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Regulated Expression of Adenoviral Vectors-Based Gene Therapies,viral vectors that can be used to treat human diseases in the clinic. Ideally, regulatable cassettes should have minimal gene expression in the “OFF” state, and expression should quickly reach therapeutic levels in the “ON” state. In addition, the components of regulatable cassettes should be non-to

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Liver-Directed Gene Therapy Using the , Transposon System,chanism. SB has been shown to mediate transposition in a variety of cells and tissues, has been used for the generation of transgenic animals and has been tested as a vector for gene therapy in several animal models of human disease. Here, we describe methods that we have developed for testing SB-me

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Generation and Functional Analysis of Zinc Finger Nucleases, correction of mutated genes directly in the chromosome. This kind of gene therapy is based on homologous recombination, which can be stimulated by the creation of a targeted DNA double-strand break (DSB) near the site of the desired recombination event. Artificial nucleases containing zinc finger D

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Joseph M. DouxCollects time tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field.Covers current and emerging methods for production of viral and non-viral gene tran

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Titlebook: Gene Therapy Protocols; Volume 2: Design and Joseph M. Doux Book 20083rd edition Humana Press 2008 DNA.Promoter.Virus.gene expression.gene

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