CRAB
发表于 2025-3-28 15:50:10
Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Genomic DNA in Eukar atrophy, cystic fibrosis, severe combined immune deficiency). Several parameters can be modified to optimize the gene modification efficiency, as described in our recent study..In this chapter we describe the main guidelines that should be followed in SFHR application, in order to increase techniqu
Infelicity
发表于 2025-3-28 22:15:49
Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotiactive psoralen-linked TFOs, their co-introduction into mammalian cells with donor nucleic acids, and the identification of cells with sequence conversion of the target site. We have emphasized details in the synthesis and purification of the oligonucleotides that are essential for preparation of re
adjacent
发表于 2025-3-29 00:53:33
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sclera
发表于 2025-3-29 06:56:49
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金丝雀
发表于 2025-3-29 09:37:27
Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus ,rapid and simple restriction enzyme site polymorphism assay to detected DSB formation at the . target sequence. Owing in part to the small molecule-based clone selection schemes conferred by . allelic knockouts, this X-linked gene has become a “classical” target model locus in mammalian cells. The r
BET
发表于 2025-3-29 15:12:55
CRISPR-Cas-Mediated Targeted Genome Editing in Human Cellshrough nonhomologous end joining (NHEJ) or through homologous recombination (HR) in the presence of an appropriate donor sequence. This RNA-guided editing tool offers greater ease of customization and synthesis in comparison to existing sequence-specific endonucleases and promises to become a highly
孵卵器
发表于 2025-3-29 19:10:34
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招惹
发表于 2025-3-29 19:59:40
Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-associated Virus
Little
发表于 2025-3-30 03:34:58
https://doi.org/10.1007/978-1-62703-761-7gene correction; gene repair; gene targeting; human genome; human pluripotent stem cells
Explosive
发表于 2025-3-30 06:26:21
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