不自然
发表于 2025-3-28 17:34:35
Evaluation of Galectin Binding by Surface Plasmon Resonance,sensitivity and low sample consumption in the Biacore enables the measurement of rapid kinetics and low affinities characteristics of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2 and -3 and their glycoside ligands using this approach.
ineffectual
发表于 2025-3-28 19:11:01
Sean R. Stowell,Connie M. Arthur,Richard D. CumminIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
非秘密
发表于 2025-3-28 23:40:52
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清澈
发表于 2025-3-29 03:07:52
Galectins978-1-0716-2055-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
floodgate
发表于 2025-3-29 09:25:20
https://doi.org/10.1007/978-3-662-04240-3ncovered intracellular galectin functions mediated by carbohydrate- and non-carbohydrate-dependent interactions. In the cytoplasm, galectins can tune intracellular kinase and G-protein-coupled signaling cascades important for nutrient sensing, cell cycle progression, and transformation. Particularly
Infant
发表于 2025-3-29 11:25:34
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COMMA
发表于 2025-3-29 15:50:07
https://doi.org/10.1007/978-3-0348-5295-1ng/purifying glycoproteins. In this method, targets (lectins and glycoproteins) are captured by multivalent ligands called target capturing agents (TCAs). The captured targets are then released and separated from their TCAs to obtain purified targets. CaRe can potentially be used as a tool to discov
generic
发表于 2025-3-29 23:13:35
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mydriatic
发表于 2025-3-30 00:35:32
https://doi.org/10.1007/978-3-642-53044-9they formed reversible noncovalent cross-linked complexes with the lectin. Binding of sulfated GAGs to Gal-3 was completely inhibited when Gal-3 was preincubated with β-lactose. Cross-linking of Gal-3 by CSA, CSC, and the bovine CSPG was also reversed by β-lactose. These findings strongly suggest th
改正
发表于 2025-3-30 05:18:10
https://doi.org/10.1007/978-3-662-05619-6bohydrate to carbohydrate epitope in these molecules leading to increased affinity. Importantly, the mechanism of binding of lectins to mucins appears similar to that for a variety of protein ligands binding to DNA. Recent results also show that high-affinity lectin–mucin cross-linking interactions