generic
发表于 2025-3-28 15:28:16
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拥护者
发表于 2025-3-28 20:10:53
Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plat evaluate the transient calcium response of the endogenous Muscarinic type 1 receptor (M1) in HEK 293 T cells. The microfluidic fabrication protocol is described as well as a methodology to monitor the cell response in real time, after stimulation with M1 agonists (e.g., carbachol) and antagonists (e.g., .irenzepine).
Flustered
发表于 2025-3-29 02:12:20
Screening for Serotonin Receptor 4 Agonists Using a GPCR-Based Sensor in Yeast,ally, the HTR.-based screen couples activation of 5-HTR. on the yeast cell surface to luciferase reporter expression. The HTR.-based screen has a throughput of one compound per second allowing the screening of more than a thousand compounds per day.
Mettle
发表于 2025-3-29 03:08:40
Imaging of Genetically Encoded FRET-Based Biosensors to Detect GPCR Activity,s manuscript are entirely genetically encoded by plasmids. Here, protocols for employing FRET-based biosensors to detect G protein activity upon GPCR activation are reported. The protocols include details on the isolation of plasmids, transfection, generation of stable cell lines with the FRET biosensors, FRET ratio imaging, and data analysis.
古文字学
发表于 2025-3-29 08:47:20
FLIPR Calcium Mobilization Assays in GPCR Drug Discovery,intracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for simultaneous concentration-dependent analysis of several receptor agonists and antagonists, useful in receptor characterization and drug discovery projects.
Mawkish
发表于 2025-3-29 14:42:03
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NADIR
发表于 2025-3-29 17:01:10
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Wordlist
发表于 2025-3-29 22:04:58
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冷淡周边
发表于 2025-3-30 03:03:08
Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples,cle membranes are excellent tools for a variety of applications such as ligand screening, engineering or even structural characterization. In this chapter, we provide protocols for the reaction set-up and efficient cell-free production of functionally folded GPCRs reaching μM concentrations in the f
Graphite
发表于 2025-3-30 04:23:02
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Crmat limiting the flexibility of data analysis. To solve this problem, we propose the use of the data curation software . (.), which integrates experimental data with metadata in a Minimum Information for Data Analysis in Systems Biology (MIDAS) format. . enables data export to different software pa