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Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Ho lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin’s laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.