Insul岛
发表于 2025-3-23 09:52:00
板凳
expository
发表于 2025-3-23 16:00:16
Amino Acid Analysis,The method described in this chapter is based on derivatization of amino acids, produced by hydrolysis of peptides or proteins, with phenylisothiocyanate. This forms phenylthiocarbamyl amino acids, which are then separated by reverse-phase high performance liquid chromatography and quantified from their UV-absorbance at 254 nm.
Anonymous
发表于 2025-3-23 21:34:52
Translating Anna Akhmatova,: How did the project originate, the co-operation with Max Hayward, because I know you worked on other projects with him?
Estrogen
发表于 2025-3-24 01:38:38
Selective Isolation of the Carboxy Terminal Peptide from a Protein,In contrast to N-termmal sequencing, several methods have been used for determination of the C-terminus. They include a thiocyanate degradation procedure (.), methods using carboxypeptidase (.), hydrazinolysis (.), and tritium labeling (.), depending on the nature of the samples.
朦胧
发表于 2025-3-24 03:17:44
Translating Penna and Cernuda,I keep as close to the original as possible, though careful to avoid a word-for-word literalism.
难取悦
发表于 2025-3-24 08:47:38
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mastoid-bone
发表于 2025-3-24 11:35:57
Protein Extraction From Plant Tissues,Plant tissues contain a wide range of proteins, which vary greatly in their properties, and require specific conditions for their extraction and purification. It is therefore not possible to recommend a single protocol for extraction of all plant proteins.
Magnificent
发表于 2025-3-24 15:23:26
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剥皮
发表于 2025-3-24 20:24:34
Optimization of Procedures; Final Steps,This chapter is intended for a later stage in purification efforts, when a method has been established, or a published method has been tried, but there may be some dissatisfaction with the overall procedure. The objectives of a scheme for the purification of an enzyme or other protein may be any or all of the following:
chastise
发表于 2025-3-25 00:43:51
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