和平主义
发表于 2025-3-27 00:05:51
Restoration of Dystrophin Protein Expression by Exon Skipping Utilizing CRISPR-Cas9 in Myoblasts Dertiated myoblasts. Herein, we describe an optimized methodology to prepare myoblasts differentiated from iPS cells by mRNA transfection of the CRISPR-Cas9 system to skip exon 45 in myoblasts, and evaluate the restored dystrophin by RT-PCR and Western blotting.
stratum-corneum
发表于 2025-3-27 03:47:50
Skipping of Duplicated Dystrophin Exons: In Vitro Induction and Assessment than may actually be induced. Unless high fidelity RT-PCR systems are used, strand slippage during annealing/elongation steps will generate normal length transcripts that are artifacts of the amplification.
offense
发表于 2025-3-27 07:07:33
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ineluctable
发表于 2025-3-27 11:39:10
neue betriebswirtschaftliche forschung (nbf)ations, and its evolution into the approach we are now familiar with. We give a more extensive history of exon skipping in particular, as it is the splice modulation approach given the most focus in this book.
resistant
发表于 2025-3-27 15:53:28
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ARCHE
发表于 2025-3-27 19:34:14
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分期付款
发表于 2025-3-27 22:39:34
S. C. Verma,K. Lan,E. Robertson type 2B; LGMD2B, and distal myopathy with anterior tibial onset; DMAT), laminin α2 chain (merosin)-deficient congenital muscular dystrophy (MDC1A), sarcoglycanopathy (e.g., limb-girdle muscular dystrophy type 2C; LGMD2C), and Fukuyama congenital muscular dystrophy (FCMD). A major challenge in exon
bonnet
发表于 2025-3-28 03:21:02
Frederic Effenberger,Natasha L. S. Jeffreydenylation signals and the methodologies relevant to their in vitro screening for efficacy and safety, including analysis of expression at the transcript and protein level of the specific target and downstream genes, and measurement of the effect on the fusion index of myotubes. The targeting of per
ALB
发表于 2025-3-28 07:39:11
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施加
发表于 2025-3-28 13:04:28
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