CHASM
发表于 2025-3-30 11:15:24
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pulse-pressure
发表于 2025-3-30 14:59:42
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objection
发表于 2025-3-30 18:39:29
Detection of Plasmid by Colony Hybridization, by considering the following problem. A scientific group wishes to clone in bacteria a particular human chromosomal gene. There would be no difficulty obtaining human DNA, cutting it with a restriction endonuclease, and ligating the many fragments obtained into vector molecules. This ligation mix c
特别容易碎
发表于 2025-3-30 22:05:14
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有帮助
发表于 2025-3-31 04:56:17
Isopycnic Centrifugation of DNA,ually associated. Cells of plants and animals contain from 1 to 100 pg of DNA per nucleus. This does not sound like a lot until one realizes that is represents 100–10,000 times as much DNA as in an . chromosome. When faced with such vast complexity, it is obviously an advantage if the DNA can be sep
才能
发表于 2025-3-31 08:54:01
Purification of Plant Ribosomal DNA,sed in the preceeding chapter. This chapter describes a modification of the procedure that enhances the separation of specific DNA sequences from main-band DNA. The method is based on the fact that actinomycin D binds preferentially to DNA molecules with a high-GC content. This . their density relat
轻快走过
发表于 2025-3-31 09:52:41
The Extraction and Affinity Chromatography of Messenger RNA,A molecules are involved in the expression of the genetic material (DNA) and are, in fact, the initial products of gene expression that go on to direct protein synthesis. Knowledge of the structure, metabolism, and mode of action of RNA in protein synthesis is therefore fundamental to our understand
过份艳丽
发表于 2025-3-31 16:06:56
Agarose Gel Electrophoresis of RNA, simple, but nevertheless achieves very reproducible results and high resolution. RNA is a polyanion and will therefore migrate toward the positive electrode in an electric field. If the migration occurs through a gel matrix of carefully chosen pore size, the mobility of the RNA molecules is related
BIDE
发表于 2025-3-31 19:59:54
In Vitro Protein Synthesis,h prokaryotic and eukaryotic systems have been developed, but for this experiment only a eukaryotic system will be considered. In 1973, Roberts and Paterson (.) demonstrated that a cell-free extract of wheat germ would faithfully translate rabbit globin mRNA and tobacco mosaic virus RNA to yield ful
AMEND
发表于 2025-3-31 22:44:59
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